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American Journal of Experimental Agriculture

American Journal of Experimental Agriculture, ISSN: 2231-0606,Vol.: 9, Issue.: 2

Original Research Article

Gene Pyramiding Improved Resistance to Angular Leaf Spot in Common Bean


G. Ddamulira1*, C. Mukankusi2, M. Ochwo-Ssemakula1, R. Edema1, P. Sseruwagi3 and P. Gepts4

1Department of Agricultural Production, Makerere University Kampala, Uganda.

 2International Centre for Tropical Agriculture, P.O.Box 6247, Kampala, Uganda.

3Mikocheni Agricultural Research Institute, P.O.Box 6226, Dar es salaam, Tanzania.

4Department of Plant Sciences, University of California, 1 shields avenue, Davis, CA 95616, USA.




Aims: The study was conducted to determine the effectiveness of pyramided genes in improving angular leaf spot (ALS) resistance in susceptible common bean cultivars.

Study Design: The experiment was set in randomized block design with three replications. 

Place and Duration of Study: The experiment was conducted at International Centre for Tropical Agriculture (CIAT) at Kawanda, Uganda in 2010-2014.

Methodology: Crosses among three Pseudocercospora griseola resistant lines of common bean (Phaseolus vulgaris) were developed. The crosses involved five inbred lines, AND277, Mexico 54, G5686 and two susceptible cultivars, K132 and Kanyebwa. The resistant lines were crossed in cascading pyramiding scheme to develop triple crosses (TC). The TC F1 and each of the resistant parents were crossed with each of the two susceptible cultivars to generate four parent crosses (FPC) and single crosses (SC), respectively. All the population developed was inoculated with 61:63 P. griseola isolate under screenhouse conditions and their reaction was elucidated.  

Results: The SC exhibited resistance segregation ratios of 15:1 and 61:3 while TC best fitted for 249:7 and 247:9 ratios. This suggested that two or three genes were present in SC and four genes in the TC. The resistance present in the three sources to ALS race 61:63 is complex; with epistatic mode of inheritance. The four genes in FPC provided more effective resistance against isolate 61:63 than two or three genes in SC.  

Conclusion: The FPC lines with combined resistance were more effective than the individual sources for transferring resistance to susceptible cultivars. Future studies needs to be conducted to determine how broad and effective combined resistances in these newly developed lines have against the variability of the ALS pathogen sampled in Uganda.


Keywords :

Complex crosses; Pseudocercospora griseola; genes; Phaseolus vulgaris.


Full Article - PDF    Page 1-12    Article Metrics


DOI : 10.9734/AJEA/2015/17833

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