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Journal of Advances in Biology & Biotechnology, 2394-1081,Vol.: 5, Issue.: 1

Short Research Article

Antioxidant, Anticancer and Antibacterial Activity of Withania somnifera Aqueous Root Extract

 

D. A. Barnes1, R. Barlow1, P. Singh Nigam1 and R. Owusu-Apenten1*

1School of Biomedical Sciences, University of Ulster, Coleraine, Cromore Road, Coleraine BT52 1SA, UK.

Article Information
Editor(s):
(1) Joana Chiang, Department of Medical Laboratory Science and Biotechnology, China Medical University,
Taiwan.
Reviewers:
(1) Abdullahi Hassan Kawo, Bayero University, Kano, Nigeria.
(2) Rodrigo Crespo Mosca, Sao Paulo University, Brazil.
Complete Peer review History: http://sciencedomain.org/review-history/12257

Abstracts

Aims: To evaluate total antioxidant capacity, anticancer activity and antibacterial effects Withania somnifera aqueous-root extracts.

Study Design: In vitro study.

Place of Study: School of Biomedical Sciences, Ulster University, UK.

Methodology: Total antioxidant capacity (TAC) of whole powder and freeze dried W. somnifera aqueous-root extracts was determined using FRAP, DPPH, Folin and ABTS assays. Anticancer activity was accessed using MDA-MB-231 breast cells and Sulforhodamine B staining for cell viability. Antibacterial activity was by disk diffusion assay with penicillin, amoxicillin and streptomycin as positive controls.

Results: The TAC for W. somnifera extract was 86, 47, 195,or 443 gallic acid equivalents per 100g dry basis (mgGAE/ 100 g) using FRAP, DPPH, Folin or ABTS assays, respectively. Corresponding TAC values for freeze dried W. somnifera aqueous-root extract were, 418, 553, 1898 or, 1770 (mgGAE/100 g). W. somnifera aqueous-root extract inhibited MDA-MB-231 cell proliferation in a dose-dependent manner with IC50 = 0.19 mg/ml (21 µM GAE). Nil antibacterial effects were detected for freeze dried W. somnifera extract (0-1 mg/ml) across six species of bacteria tested.

Conclusion: Withania somnifera root water extract showed significant antioxidant and anticancer activity for MDA-MB-231 breast cancer cells but no antibacterial activity under the conditions of this study.

Keywords :

Withania somnifera; antioxidant capacity; anticancer; antibacterial.

Full Article - PDF    Page 1-6

DOI : 10.9734/JABB/2016/22523

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