Journal of Advances in Biology & Biotechnology, 2394-1081,Vol.: 5, Issue.: 1
Antioxidant, Anticancer and Antimicrobial, Effects of Rubia cordifolia Aqueous Root Extract
R. Barlow1, D. Barnes1, A. Campbell1, P. Singh Nigam1 and R. Owusu-Apenten1* 1School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, UK.
R. Barlow1, D. Barnes1, A. Campbell1, P. Singh Nigam1 and R. Owusu-Apenten1*
1School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, UK.
(1) Anil Kumar, Professor & Head (Chair), School of Biotechnology, Devi Ahilya University, Madhya Pradesh, India.
(1) Ayona Jayadev, All Saints’ College, Kerala, India.
(2) Sahar Mohamed Kamal Shams El Dine, Ain Shams University, Cairo, Egypt.
(3) Ahmed Hegazi, National Research Centre, Egypt.
Complete Peer review History: http://sciencedomain.org/review-history/12260
Aims: To evaluate the total antioxidant capacity (TAC) of Rubia cordifolia root extracts, to test anticancer activity against MDA-MB-231 breast cancer cell lines, and to evaluate antimicrobial activity of the same extract versus six Gram-positive and negative bacteria.
Study Design: In vitro.
Place of Study and Duration: School of Biomedical Sciences, Ulster University, July 2014-Sept 2015.
Methodology: TAC was tested using ABTS, DPPH, FRAP and Folin assays and values were expressed as mg-gallic acid equivalents per 100 g (GAE/100 g) of sample. Anticancer properties were examined against MDA-MB-231 breast cancer cell lines using Sulforhodamine B assay. Antimicrobial activity was examined using a disk diffusion assay with three Gram-positive (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi) bacteria.
Results: TAC of dry extracts of Rubia cordifolia ranged from 523±43 to 4513±208 (mg GAE mg/100 g) depending on the method of analysis, ABTS> FRAP> Folin > DPPH methods. R. cordifolia dry extract showed cytotoxicity against MDA-MB-231 with IC50 = 44 µg/ml or 5.1µM GAE. No antimicrobial activity was observed against the three Gram-positive, or three Gram-negative bacterial species using the water extract or R. cordifolia.
Conclusion: R. cordifolia aqueous extract possess high total antioxidant capacity but values depend on the method of analysis. R. cordifolia extract inhibits MDA-MB-231 breast cancer cells proliferation but nil anti-bacterial activity was observed for three Gram-positive and three Gram-negative bacterial strains tested.
Manjistha; Rubia cordifolia; antioxidant; antimicrobial; MDA-MB-231; breast cancer; ABTS; DPPH; FRAP.
Full Article - PDF Page 1-8
DOI : 10.9734/JABB/2016/22625Review History Comments