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Journal of Applied Life Sciences International, 2394-1103,Vol.: 4, Issue.: 3

Original-research-article

Antioxidant Activity of Curcumin and Neem (Azadirachta indica) Powders: Combination Studies with ALA Using MCF-7 Breast Cancer Cells

 

T. K. N. Cheung1, P. Singh Nigam1 and R. Owusu-Apenten1*

1School of Biomedical Sciences, Ulster University, Cromore Road, Coleraine BT52 1SA, UK.

Article Information
Editor(s):
(1) Shahira M. Ezzat, Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Egypt.
Reviewers:
(1) Upendarrao Golla, Indian Institute of Science Education and Research, Bhopal, India.
(2) Patricia Gonzalez-Barranco, Universidad Autonoma de Nuevo Leon, Mexico.
(3) Intan Soraya Che Sulaiman, Universiti Putra Malaysia, Malaysia.
(4) Mary Ann Foglio, Universidade Estadual de Campinas, Brazil.
Complete Peer review History: http://sciencedomain.org/review-history/12881

Abstracts

Introduction: Breast cancer is the most common cancer affecting women globally. The essential fatty acid α-Linolenic acid (ALA) and its oxidation products inhibit cancer cell proliferation. The effect of natural antioxidants on ALA anticancer effects has not been well characterized. 

Aims: To assess the effect of curcumin and neem leaf powder extract, on ALA cytotoxicity activity towards MCF-7 breast cancers.

Study Design: In-vitro testing.

Methodology: Antioxidant activity of neem extract and curcumin were evaluated using, four assays: Total phenolic content, Ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay or 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay. Cytotoxicity activity was assessed using MCF7 cells grown in DMEM (+10% FBS) and evaluated using Sulforhodamine B colorimetric assay for cell cytotoxicity.

Results: Curcumin and neem leaf extract had significant antioxidant power, and values varied between the four assays. Treatments of MCF7 cells with ALA, (0-500 µM) curcumin (0-50 µM), and neem leaf extract (0-88 µM) individually produced a concentration-dependent decrease in MCF-7 cell viability. Combination treatments using ALA with curcumin and ALA with neem were significantly less effective compared to individual treatments.

Conclusion: Combinations studies indicate that the natural antioxidants curcumin and neem reduce the inhibitory effect of α-Linolenic acid towards MCF-7 breast cancer cells.

Keywords :

Antioxidant; total phenols; curcumin, neem (Azadirachta indica), linolenic acid; MCF-7 cell; anticancer.

Full Article - PDF    Page 1-12

DOI : 10.9734/JALSI/2016/22273

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