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British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 13, Issue.: 6

Original-research-article

A New Centrifugation Method for the Improvement of Platelet-rich Fibrin Products: A Preliminary Study

 

Mustafa Tunali1*, Hakan Özdemir2, Zafer Küçükodacı3, Serhan Akman4, Elif Öncü5, Mustafa Aydınbelge6, Melek Akman7 and Erhan Firatli8

1Department of Periodontology, Gulhane Military Medical Academy, 34618 Istanbul, Turkey.

2Department of Periodontology, School of Dentistry, Osmangazi University, 26000 Eskisehir, Turkey.

3Department of Medical Pathology, Gulhane Military Medical Academy, 34618 Istanbul, Turkey.

4Department of Prosthodontics, School of Dentistry, Selcuk University, 42250 Konya, Turkey.

5Department of Periodontology, School of Dentistry, Necmettin Erbakan University, 42050 Konya, Turkey.

6Department of Pedodontics, School of Dentistry, Erciyes University, 38039 Kayseri, Turkey.

7Department of Endodontics, School of Dentistry, Necmettin Erbakan University, 42050 Konya, Turkey.

8Department of Periodontology, School of Dentistry, Istanbul University, 34303 Istanbul, Turkey.

Article Information

Editor(s):

(1) Alex Xiucheng Fan, Department of Biochemistry and Molecular Biology, University of Florida, USA.

Reviewers:

(1) Neha Saksena, Shree Guru Gobind Singh Tricentenary University, India.

(2) Jorge Paredes Vieyra, Universidad Autonóma de Baja California, Tijuana, Mexico.

Complete Peer review History: http://sciencedomain.org/review-history/13088

Abstracts

Introduction: Nowadays, there are many articles about Platelet Rich Plasma/Platelet Rich Fibrin families. A novel platelet-rich product called titanium prepared platelet-rich fibrin (T-PRF) has stronger and thicker fibrin than that of the classic glass tube prepared platelet-rich fibrin. Strong fibrin structure is important to extend the time for resorption of fibrin in-vivo, and increase the release time of growth factors.

Objective: In this preliminary study of a new centrifugation method, we aimed to change the direction of fibrin formation during the platelet aggregation, and make T-PRF much denser and more resistant. According to our hypothesis, it can make it possible to use in guided bone, and guided tissue regeneration more successfully.

Methods: Blood samples of 10 healthy male volunteers were collected, and four 10ml blood samples, one for each of four groups, were transferred to a Ti tube from each volunteer. The first group was centrifuged for a 20-minute period clockwise (T-PRF group), and the other groups were centrifuged for a total of 20 minutes with two-minute (2min MT-PRF group), five-minute (5 min MT-PRF group), and ten-minute (10min MT-PRF group) periods clockwise and counter-clockwise.

Results: By hematoxylin and eosin stain, the 10min MT-PRF group showed a better-organized network with continuous integrity compared to the other groups. With the immunofluorescent staining, fibrin seemed thicker and better organized in the 10 min MT-PRF group. SEM examination showed more complex and denser fibrin clusters in the 10 min MT-PRF group than the other groups.

Conclusion: This pilot study defines 10 min MT-PRF as a new autogenous product with superior fibrin network. Our results showed that, fibrin formation was made more organised and denser with 2-way direction centrifugation.

Keywords :

Tissue engineering; biomaterial(s); centrifugation; scanning electron microscopy (SEM); wound healing; blood; histomorphometry.

Full Article - PDF    Page 1-10

DOI : 10.9734/BJMMR/2016/23939

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