International Journal of Biochemistry Research & Review, ISSN: 2231-086X,Vol.: 12, Issue.: 1
Anti-diabetic Effect of the Methanolic Leaf Extract of Axonopus compressus (P. Beauv) in Alloxan Induced Diabetic Rats
Ukegbu Chimere1, Odiba Arome1*, Edeke Affiong1, Anunobi Ogechukwu1 and Chukwunonyelum Ike1 1Department of Biochemistry, University of Nigeria, Nsukka, Nigeria.
Ukegbu Chimere1, Odiba Arome1*, Edeke Affiong1, Anunobi Ogechukwu1 and Chukwunonyelum Ike1
1Department of Biochemistry, University of Nigeria, Nsukka, Nigeria.
(1) Chunying Li, Department of Biochemistry and Molecular Biology Wayne State University School of Medicine, Detroit, USA.
(1) Wycliffe Arika, Kenyatta University, Kenya.
(2) Parineeta Samant, MGM Medical College, Navi-Mumbai, India.
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Aims: This study is a preliminary step in investigating the anti-diabetic effect of the methanolic extract of the leaf of Axonopus compressus (P. Beauv) in alloxan-induced diabetic rats.
Study Design/Methodology: Intraperitoneal (i.p.) injection of alloxan monohydrate at the dose of 180 mg/kg b.w was carried out to induce diabetes in the rat models. Different doses (250, 500 and 1000 mg/kg b.w.) of the extract was administered per oral through gastric gavage to the alloxan induced diabetic rats, and 2 mg/kg b.w. of glibenclamide was used as the standard drug, and only tween 20 solution (10 ml/kg) given to the negative control group. AccuCheck Active® (an auto-analyzer) glucose kit was used to assay the effects of the P. Beauv extract and the standard drug on the fasting blood glucose level at 0, 1, 3 and 6 hr(s) using the blood collected through the snip made in the tail of the rats.
Results: The different doses of 250, 500 and 1000 mg/kg b.w. produced a significant (p < 0.05) reduction in the blood glucose level of the alloxan-induced diabetic animal models in the degree of 31.5%, 19.8% and 24.5% respectively, compared to the negative control group and this was time dependent (at the end of the 6th hour, although a decrease in the blood glucose was also noticed at the 1st and 3rd hours). 2 mg/kg glibenclamide decreased the blood glucose levels by 69.9% while the blood glucose level of the negative control group increased by 15.2% at the 6th hour.
Conclusion: It is obvious from the above findings that Axonopus compressus deserves further investigations into the active ingredients and structural allucidation to validate the anti-diabetic property it possess.
Alloxan; Axonopus compresus; diabetes; glibenclamide.
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