Dr. Héctor Manuel Mora Montes
Héctor Manuel Mora Montes (42 years old) studied the University degree in Clinical Chemistry (1993-1997) in Universidad Veracruzana, where we received the award as best undergraduate student, with a final average mark 9.8/10. Soon after finishing his basic formation, we worked as Clinical analyst (1997-2000) in Centro de Especialidades Médicas del Estado de Veracruz “Doctor Rafael Lucio”. Veracruz, During this post, he obtained valuable experMieénxceicoa.s clinical microbiologist, carrying out experiments in the typing of fungi, bacteria and mycobacteria; and developed a system for the differentiation of Candida species, based on in-house zymograms for carbohydrate assimilation, and colony morphology in corn meal agar. In the immunology department, he received training in Western blotting and ELISA for the detection of virus, hormones, bacteria and cancer markers. In the haematology area, we developed skills to identify different classes of white and red blood cells associated with diverse diseases, which was reinforced by a Diplomat in Haematology that finished in 2000. Then, he moved to basic science and started a MSc program in Genetics and Molecular Biology (2000-2002) in Departamento de Genética y Biología Molecular del Centro de Investigación y Estudios Avanzados del I.P.N., México, D.F. His work was focused in the biochemical and molecular characterization of C. albicans mannosidases, gaining experience in the purification and biochemical characterization of soluble proteins, and becoming familiar with standard recombinant DNA technology, and nucleic acid analysis. He continued with a PhD program in Experimental Biology (2002-2006) in Universidad de Guanajuato, Guanajuato, México, with an experimental work focused in the same topic, C. albicans mannosidases. During this period he complemented his molecular biology and biochemical skills with new techniques, including biochemical characterization of membrane-bound proteins, purification and analysis of carbohydrates using high-performance anion-exchange chromatography, generation of polyclonal antibodies, intracellular protein localization using ultracentrifugation techniques, fungal gene isolation and disruption, characterization of mutant phenotypes with defects in the glycosylation pathways, and heterologous expression of proteins. It is noteworthy to mention that his experience in biochemistry was translated in the development of a new and innovative method for in situ determination of glycosyl hydrolase activities. He received the award as best PhD student and the “Summa Cum Laude” distinction from Universidad de Guanajuato. During the PhD studies, he has a research-training visit at the Aberdeen Fungal Group, University of Aberdeen, Aberdeen, U.K., under supervision of Prof. Neil A. R. Gow (2005). In this period, he learnt to generate C. albicans null mutants using the mini-ura-blaster system, and to test the N- linked and O-linked mannosylation status using in situ zymograms and TLC assays, respectively. His performance and experience where noticed by Prof. Gow whom offered him a post as postdoctoral research fellow, which was accepted and lasted until 2009. During this post, he further enhanced his experience through the assimilation of relevant methods to study the infectious disease immunology. To achieve this goal, he performed a three-month research training in Radboud University Nijmegen Medical Centre (Nijmegen, The Netherlands, 2007) under supervision of Prof. Mihai G. Netea, which allowed him to learn the isolation and manipulation of several human immune cells (peripheral blood mononuclear cells, macrophages and dendritic cells).